DNA Barcoding of Fishes is a comprehensible book that explains the role of innovative field of DNA barcoding in species identification and phylogenetic analysis. Written by experts in the field, this book provides a comprehensive overview of the principles, techniques, and applications of DNA barcoding in biodiversity research. This book is equally useful for analysis of other amplicon sequence-based markers used for species identification, phylogenetic and evolutionary analysis. The book begins by introducing the concept of DNA barcoding and its historical development, highlighting the need for a reliable and efficient method for species identification. It then traverses through the process of exploration of waterbodies for sample collection, and fish species identification by traditional taxonomy. The core of the book lies in step by step explanation of DNA isolation, polymerase chain reaction, Sanger’s sequencing, DNA sequence submission in NCBI, species delimitation, genetic distance and phylogenetic analysis. Readers will appreciate the book’s accessible language and clear explanations, making complex scientific concepts understandable to a broad audience. Case studies and examples from real-world research further illustrate the potential and versatility of DNA barcoding in the study of biodiversity. Whether you are a student, researcher, or simply interested in the intersection of genetics and ecology, “DNA Barcoding” offers a compelling exploration of a cutting-edge scientific technique that is reshaping our understanding of the natural world. It inspires readers to contemplate the future possibilities and ethical considerations associated with this powerful tool, making it a valuable resource for anyone seeking to stay informed about the latest advancements in molecular biology and biodiversity science.
Dr. Mahender Singh Principal Scientist ICAR-National Bureau of Fish Genetic Resources Dr. Mahender Singh possesses more than 24 years research experience in several areas including DNA barcoding of fishes, integrative taxonomy, population genetics, phylogenetics and evolutionary genetics, genome & transcriptome analysis, protein modeling and genomic database designing. He has discovered more than 10 new fish and fish pathogen species, generated over two thousand DNA barcodes of 447 marine and 202 fresh water fish species. He has published over 80 research papers, guided/being guided 8 Ph.D. scholars and undergone specialized training at Institute of Aquaculture, University of Stirling, Scotland, United Kingdom.
Ashish Sahu Ph.D. Research Scholar Kerala University of Fisheries and Ocean Studies, Cochin, Kerala Ashish Sahu is currently pursuing Ph.D. in Fisheries Resource Management from KUFOS, Kochi, Kerala under the guidance of Dr. Mahender Singh (Principal Scientist). He is working on the Integrative Taxonomy of fishes, metabarcoding and environmental DNA (eDNA).
Dr. Uttam Kumar Sarkar Director ICAR-National Bureau of Fish Genetic Resources Dr. Uttam Kumar Sarkar is presently working as a Director, ICAR-National Bureau of Fish Genetic Resources. He possesses more than 28 years research experience in several areas including conservation biology, gene banking and contributed significantly in exploring, evaluating and characterizing fish biodiversity including DNA bar-coding of fishes. Currently, developed climate resilient adaptation techniques and developed methods/models on selected fishes in relation to climate change and vulnerability of fish and aquatic ecosystem. He has published over 230 research papers, 5 books, 6 bulletins, 12 databases, etc. He has also guided 12 Ph.D. scholars and undergone specialized training at Cornell University, Ithaca, New York, USA.
1. An Introduction to DNA Barcoding ............................................. 1
2. Aquatic Biodiversity Exploration and Documentation for Sustainable Management ........................................................ 13
3. Fish Species Identification by Morphological Characters ....... 21
4. Methodology of DNA Isolation .................................................... 33
5. DNA Amplification by Polymerase Chain Reaction ................ 45
6. DNA Sequencing by Sanger’s Method........................................ 53
7. Sequence Submission in NCBI ...................................................... 67
8. Species Delimitation and Calculation of Genetic Distance ..... 73
9. A Glimpse of Phylogenetic Analysis ............................................ 81
10. Potentials of Integrative Taxonomy for Species Discovery, Documentation and Creation of Molecular Signature ............ 91
11. Metabarcoding: A Non-invasive Tool for Biodiversity Monitoring ......................................................................................109
12. Hidden Species Uncovered: The Crucial Role of DNA Barcoding in Discovery and Authentication of Fish Species....................................................................................125
