Helping learners (PG students, Food analysts, and pharmaceutical Industries) with Method Development, validation, troubleshooting using real world examples, and appropriate experimental layout is the main goal of this work. To ensure that all parties involved should fully grasp the concepts and for this purpose real data has been given along with a step-by-step solution. Practical data has been used to illustrate theoretical plates, plate height, resolution, selectivity, and the Van Deemter equation. In order to initiate NABL accreditation in different laboratories across the nation, deliberate incorporation of ICH (International Consortium Harmonization), SANTE guidelines and other regulatory compliances has been given in this book.
Dr. Javid Ahmad Sofi Professor Soil Science SKUAST-K, Faculty of Horticulture Shalimar-190025, Srinagar, J&K.
1. Introduction to Chromatography ..................................................1
2. Types of Chromatography............................................................23
3. Thin Layer Chromatography........................................................35
4. Liquid-Liquid Partition Chromatography..................................47
5. Adsorption Chromatography.......................................................53
6. Column Chromatography ............................................................59
7. Gel Chromatography or Gel Permeation Chromatography....69
8. Ion exchange Chromatography....................................................77
9. High Performance (Pressure) Liquid Chromatography...........95
10. Gas Chromatography .................................................................137
11. Troubleshooting ..........................................................................183
12. Analytical Technologies in Food Safety....................................205
13. Method Validations in Pesticide Residue.................................247
