Protocols In Livestock Genome Analysis

Protocols In Livestock Genome Analysis

by Anuj Chauhan, Subodh Kumar Et. All
  • ISBN: 9789381226841
  • Binding: Hardbound
  • Year: 2014
  • Language: ENGLISH
PRICE:  US $ 90.00
Add to cart

The book Protocols in Livestock Genome Analysis Presents the Fundamental principle and detailed protocols of laboratory techniques with an adequate amount of thought been applied towards making it simplistic and effective. The book has been structured into seventeen chapters. It begins with Chapter 1, givingreaders some basic safety and precautions while working in molecular genetics laboratory that is exposed to hazardous agents. Chapter 2, 3 and 4 explains the most basic and fundamental procedures in molecular genetics laboratory i.e. isolation of genomic DNA, plasmid DNA, total RNA which are prerequisite for researchers conducting downstream genetics research like gene amplification or expression. Chapter 5 deals with quantification of DNA and RNA with specific examples. Chapter 6 deals with technique of polymerase chain reaction for amplification of a specific DNA sequences while Chapter 7 focuses on various variants of polymerase chain reaction that have been developed over the years for specific applications. Chapter 8 discusses the reverse transcriptionpolymerase chain reaction which is important prerequisite for gene expression studies and genetic characterization. Chapter 9 describes at length, agarose gel electrophoresis for the separation of nucleic acids and their analysis. Chapter 10describes single strand conformation polymorphism, a technique that is capable of identifying most sequence variations in a single strand of DNA. Chapter 11 details the restriction digestion of DNA molecules by restriction endonucleases. Chapter 12 describes real-time PCR that can detect their accumulation and quantify the number of substrate DNA molecules present in starting sample. Chapter 13 discusses the method of chromatin immunoprecipitation, a very important technique for elucidating the transcriptional regulation of gene expression. Chapter 14 deals with detailed analysis of microsatellite repeat sequences along with statistical tools for data analysis. Chapter 15 explains the very important procedures of DNA ligation, competent cells preparation and transformation involved in cloning of any desired DNA sequence. Chapter 16 describes the set of steps for retrieving QTL & SNP information from online Animal QTL databases. Chapter 17 provides a simple understanding of DNA microarrays. Chapter 18 details the in situ hybridization method for localization of genetic material. Chapter 19 provides the recipe for preparation of buffers and solutions required for conducting various procedures outlined in previous chapters.

Veterinary Research Institute, Izatnagar since past five years. The author is involved in post graduate teaching and research in the institute and has successfully completed projects on understanding key transcriptional factor binding sites in DNA governing immune response in cattle and gene therapy procedures in cattle results of which have been presented at prestigious scientific forums. He is currently involved in two ambitious projects aimed at finding predisposing DNA markers for tuberculosis and brucellosis in cattle. He is also associated with DBT sponsored program for upliftment of SC/ST communities through introduction of improved swine germplasm.

Dr. Subodh Kumar is Senior Scientist at the Division of Animal Genetics in the Indian Veterinary Research Institute, Izatnagar. He has research and teaching experience of more than 15 years in the field of Animal Genetics and Breeding and has guided numerous Post Graduate and Doctoral degree students. He has 41 research papers and 104 GenBank submissions to his credit. He has successfully completed 8 research projects in various capacities. Presently, he is instrumental in discovering and exploring genes that are responsible for influencing sperm motility in cattle by the fund provided by the prestigious National Agricultural Innovation Project (NAIP).

Dr. Arvind Sonwane is a Scientist (Senior Scale) at the Division of Animal Genetics in the Indian Veterinary Research Institute, Izatnagar for the past five years. With specialization in field of Animal Biotechnology, he is involved in post graduate teaching and research in the institute. He has successfully completed a key research project on Gene therapy in cattle with promising results and was also associated in projects on miRNAs profiling and finding transcription factor binding sites. He is currently involved in an ambitious project aimed at finding predisposing DNA markers for brucellosis in cattle. The author has been a key resource person facilitating the post graduate research of a number of master’s and doctorate students passing out from the division.

Dr. Deepak Sharma is Head, Division of Animal Genetics at the Indian Veterinary Research Institute, Izatnagar. He possesses vast experience in the field of Animal as well as Avian Genetics research and teaching. He has 130 refreed research publication and 8 books to his credit. He has also been instrumental in developing of three improved varieties of guinea fowl viz. Shwetambari, Chitambari, Kadambari, and two of egg type germplasm. He is recipient of prestigious ICAR Team Research Award for Biennium 1997-98 and Prof. Ramraksha Kiran Shukla Award. He is also member of several esteemed committees including DST’s National Awards committee for R&D Efforts in Industry and ISI committee for Poultry Housing and Management and Feed, ISI, New Delhi. He also acts as Editor to some prestigious research journals of the subject.

  • Laboratory Safety and Precautions
  • Isolation of Genomic DNA
  • Isolation of Plasmid DNA
  • Isolation of Total RNA
  • Quantification of Nucleic Acids
  • Polymerase Chain Reaction (PCR)
  • Variants of Polymerase Chain Reaction
  • Reverse Transcription- Polymerase Chain Reaction (RT-PCR)
  • Agarose Gel Electrophoresis
  • Single Strand Conformation Polymorphism (SSCP)
  • Restriction Digestion
  • Real Time PCR
  • Chromatin Immunoprecipitation Assay
  • Microsatellite Analysis
  • DNA Ligation, Competent Cells Preparation and Transformation
  • Retrieving QTL and SNP Information from Databases
  • DNA Microarray
  • In situ Hybridization
  • Preparation of Required Buffers and Solutions