Efficient control of diseases of animals and ensuring a particular animal health status require appropriate diagnostic measures. Laboratory diagnosis concentrates on both identification of the agent (antigen identification) and measurement of the immune response against the pathogen (antibody detection). However, even when applying both approaches, the diagnosis of certain infections is ineffective and too cumbersome, for example during latent infections in BHV1, when there is little chance of detecting the virus DNA by conventional diagnostic techniques. With the advent of genetic engineering or biotechnology, a galaxy of new methods/techniques is available to the diagnosticians and animal health officers to diagnose the infectious disease of diverse pathogen origin promptly, accurately and economically. The latest methods offer specificity and sensitivity well above the conventional methods providing the opportunity of taking more effective control measures due to quick availability of the results. In the past 30 years, veterinary diagnosticians have incorporated new molecular techniques such as the PCR, real time PCR, LAMP, other nucleic acid based technologies, microarray, viral metagenomics, nanotechnology, lateral flow assay, biosensors, flow cytometry etc for prompt, accurate, specific and sensitive detection of pathogens besides a number of other potential applications. Although, conventional diagnostic assays are still used routinely, new molecular techniques have broadened the scope of veterinary and medical diagnostics and provided powerful new tools that enabled the rapid and specific diagnosis of animal diseases with a high degree of certainty. This book “Advanced Molecular Diagnostic Techniques: Principles and Applications” is written based on the most relevant and latest informations so that everybody can read and understand it quickly, easily and properly. This book is useful for scientists, teachers, students, officers, diagnosticians, laboratory technicians and medical personnel associated directly or indirectly with research, teaching, training, extension, diagnosis, epidemiology and control of diseases of bacterial and viral origin. This book is highly informative to the Life Sciences students and other enthusiastic readers appearing in the various competitive examinations such as JRF, SRF, IAS, CSIR, NET, ARS, ICAR, IFS, IPS, Lectureship etc. Finally, all can enrich their knowledge on any aspects of diagnostic techniques from the book and can clarify any doubts and concepts very easily and confidently.
Dr. Sukdeb Nandi was born on 2nd January, 1963 at Mamudpur Village, District Hooghly, West Bengal. Dr Sukdeb Nandi graduated in Veterinary Science and Animal Husbandry in 1986 from Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, W.B. and ranked first class third position. He did his Master Degree and Ph. D. in Veterinary Virology from Deemed University, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P. in 1988 and 1992 respectively. He had carried out research on important viral diseases of animals namely Rabies, FMD, Sheep Pox, Goat Pox, Bluetongue, Infectious Bovine Rhinotracheitis, Classical Swine Fever Virus, Canine Parvovirus, Canine Distemper Virus, Infectious Canine Hepatitis Virus and Avian Influenza or Birdflu. He had also organized a DBT sponsored training programme entitled “Serological and PCR based Diagnosis of Economically Important Infectious Diseases of Domestic Animals” for the mid-career Scientists, Teachers, Assistant and Associate Professors in 2009. He was awarded with DBT sponsored National Biotechnology Associateship and carried out research on “Molecular Cloning, Characterization and Expression of Adenosine kinase related Cyclophilin homologue of L. donovani” at Indian Institute of Chemical Biology, Jadavpur, Kolkata -11 from December, 95 to December, 96. He was also deputed to United Kingdom (U.K.) to undergo ‘Transfer of Molecular Biology Training’ (TOMBIT) to carry out research on “Molecular basis of Assembly and Antigenicity of Foot and Mouth Disease Virus Capsid” under the guidance of Dr A. M.Q. King and Dr G. Belsham at Institute for Animal Health, Pirbright, London, U.K. from September, 1998 to May, 1999. He had also attended a FAO sponsored international training on ‘Advanced Biorisk Training’ at Australian Animal Health Laboratory (AAHL), Geelong, Australia in 2011 under the guidance of Dr Chris Morrissy. He had widely traveled different places in United Kingdom, Scotland, Netherland, France and Australia. Presently, he is working as Principal Scientist at Centre for Animal Disease Research and Diagnosis, Indian Veterinary Research Institute, Izatnagar, U.P. He is actively involved in the diagnosis of viral diseases of animals and shouldering the responsibility of BSL-3 laboratory for routine surveillance of birdflu samples from different parts of the country. He is also the author of ‘Manual of Viral Disease Investigation’ – a book very much useful for the scientists, teachers, students, diagnosticians and others who are working in this area. Other books in his credit are Veterinary Virology : at a glance, Immunology – at a glance, Animal Cell Culture and Virology, Avian influenza or Birdflu, Rabies : a killer disease, Veterinary Public Health – at a glance, Poultry Diseases – at a glance, Infectious diseases of animals : Their identification and Treatment and Zoonotic diseases.