In spite of achieving the highest milk production, the performance of Indian cattle has been extremely poor and average milk yield is far below the yield in other countries. Poor performance of indigenous cattle is due to more populations of non-descript animals and inadequate exploitation of their productivity because of poor nutritional and managemental factors. In spite of more number of non-descript animals, certain breeds of Indian cattle (Bos indicus) yields good amount of milk and also well adopted for harsh Indian climate. Various selection methods based on different measures of persistency have been applied in the past. The molecular genetic markers in breeding programs could make selection more precise and efficient. Some of these markers are called candidate genes like GH genes, which are usually selected because of their biological significance on the quantitative traits of interest. The book entitled “Lactation Persistency and its Association with Bovine Growth Hormone Gene authored by “Shweta Sachan and I D Gupta” has been designed to meet the needs of the research scholars, teachers, and scientists associated with Animal Genetics and Breeding. The work described in this book is the research work of M.V.Sc. dissertation entitled “Genetic analysis of lactation persistency and its association with genetic variants of bovine growth hormone gene in Sahiwal cattle”. The content in the book represents an attempt to summarize and consolidate a considerable amount of information relative to the lactation persistency and its association with bovine growth hormone gene. Each section of the book provides a structured approach to learning by covering the topics in a uniform and systematic format.
Dr. Shweta Sachan • Born on 15th August, 1986 at Kanpur Nagar, Uttar Pradesh, India • B.V.Sc. & A.H. from College of Veterinary Science, DUVASU, Mathura, Uttar Pradesh, India in the year 2016 • M.V.Sc. in the Animal Genetics and Breeding from ICAR-NDRI, Karnal, Haryana, India in the year 2018 • Persuing Ph.D. in the Animal Genetics and Breeding from ICARIVRI, Izatnagar, Uttar Pradesh, India
Dr. Shweta Sachan born in Kanpur, a city in Uttar Pradesh in 1986, Dr. Sachan had her schooling in the same city from Shri Sanatan Dharam Saraswati Girls Inter College and graduated in Bachelor of Veterinary Science and Animal Husbandry (B.V.Sc. & A.H,) in the year 2016 from the College of Veterinary Science and Animal Husbandry, DUVASU, Mathura, securing a first division. Dr. Sachan qualified ICAR- Junior Research Fellowship (JRF) for Maters Degree Programme and completed her Master of Veterinary Science (M.V.Sc.) in Animal Genetics and Breeding from ICAR-National Dairy Research Institute (NDRI), Karnal, Haryana, in the year 2018 with first division. Dr. Shweta Sachan secured 4th place in ICAR-Indian Veterinary Research Institute (IVRI), Izatnagar, Uttar Pradesh entrance examination for Ph.D. Degree Programme. Therefore, she has been awarded Institutional Fellowship. Presently, she is pursuing Ph.D. Degree in Animal Genetics and Breeding at ICAR-IVRI, Izatnagar, Uttar Pradesh. Dr. Sachan has qualified ICAR-National Eligibility Test (NET) and ICAR-Senior Research Fellowship (SRF) in the year 2018. Her research work on the genetic analysis of lactation persistency in dairy animal received considerable acclaim. She has three research articles; one review article accepted in peer reviewed journals and published four magazine articles. Dr. Sachan attended three conferences and more than ten web conferences. She is lifetime member of Animal Nutrition Association (ANA), Indian Poultry Science Association (ANSI) and Society of Conservation of Domestic Animal and Bio-diversity (SOCDAB). She has won various quizzes and awards in various competitions.
Dr. I.D. Gupta Principal Scientist Molecular Genetics Lab, Dairy Cattle Breeding Division ICAR-National Dairy Research Institute, Karnal-132001 (Haryana) Current Research Area Animal Cytogenetics & Molecular Genetics Major Research Accomplishments • Cytogenetic screening of pesticides, insecticides and antihelmenthic drugs and their effect on chromosomal profile of sheep to assess mutagenecity of these compounds using various in vitro and in vivo cytogenetic tests. • Estimation of Y chromosome length variation and chromosomal abnormalities using lymphocyte culture technique and their relationship with semen quality and temperament of the crossbred bulls. • Identification of DNA polymorphism of production, mastitis, heat shock proteins and fertility related candidate genes was explored in Sahiwal, Tharparkar, Karan Fries and Jersey Crossbred Cattle. • PCR-RFLP analysis and SNP mining was carried out in Growth Hormone gene (Bovine Somatotropin), kappa Casein, beta casein, beta Lactoglobulin, Leptin; Lactoferrin; PIT-1; Prolactin; Interleukin-8 (IL-8), Bovine Forebrain embryonic zinc finger like gene (FEZL); Caspase activating recruitment domain 15 gene (CARD 15); Calgranulin gene (S100A8); Factor XI deficiency gene (FXI) and Toll-like receptor 4 gene (TLR 4), Cluster of Differentiation 14 (CD 14) gene, Uncoupling Protein-1 ( UCP1), heat shock protein beta-1 (HSPB1), Heat shock 70 protein (HSPA1A), Heat shock protein 70 (HSPA2), ATPase, Na+/K+ transporting, alpha 1 polypeptide (ATP1A1), ATPase, Na+/K+ transporting, beta 2 polypeptide (ATP1B2), Osteopontin (OPN gene in dairy cattle and buffaloes. Student Guided • M.V.Sc./M.Sc. ->15 • Ph. D. ->10 Awards • Jawahar Lal Nehru Award - 1991 (ICAR) for best Ph.D. Thesis • Fellowship Award -1999 • Various other awards in conferences and symposia Publications • Research article- >100 • Review article- >10 • Magazine article- >10
Introduction........................................................................................1
2. Review of Literature .........................................................................5
3. Materials and Methods...................................................................37
4. Results and Discussion...................................................................51
5. Summary and Conclusion.............................................................63
6. Bibliography .....................................................................................67
Appendix..........................................................................................81