This manual “The Manual of Biocontrol Agents” it’s the Entomology/ Plant Pathology course/practical materials used for the U.G., M.Sc. and Ph.D. students under the teaching programme in Agricultural Universities. This manual is very helpful to understand the beneficial organism rearing programme during the laboratory. This compilation of manual to improve your practical knowledge. I am very confident that the manual would be a very good source of practical material for the students of Agriculture and others.
Mr. Dwarka Research Scholar, Agricultural Entomology is at Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur, Madhya Pradesh, India. He did his post-graduation in Agricultural Entomology from Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur, Madhya Pradesh, India. He has qualified ICAR-NET and received different awards from different professional societies and institutes. He has published many more research papers, review paper, book chapter, popular articles and abstracts publications to his credit.
Ms. Babli Research Scholar, Plant Pathology Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior, Madhya Pradesh, India. She did her post-graduation in Plant Pathology from Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur, Madhya Pradesh. Graduation from Rafi Ahmed Kidwai College of Agriculture, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior, Madhya Pradesh, has been awarded in the discipline of Plant Pathology from different professional societies and institutes. She has added many research papers and book chapters, popular articles, abstracts and presented research in national and international scientific forum with good publications to her credit.
1. Mass Production Procedure of Entomopathogenic Nematodes (EPNs) ............................................................................ 1
2. Mass Production Procedure of Trichogramma spp. and Trichogrammatoidea ................................................................... 7
3. Procedure for Mass Production of Bracon Hebetor .................... 15
4. Procedure for Mass Production of Goniozus Nephantidis ......... 17
5. Procedure for Mass Production of Chelonus Blackburni ............ 19
6. Mass Production of Trichoderma Viridae ..................................... 21
7. Mass Production of Biological Control Agent on Potato Dextrose Agar (PDA) ........................................................ 23
8. Mass Production of Entomopathogenic Fungi Beauveria Bassiana ............................................................................ 27
9. Production of Nuclear Polyhedrosis Virus (HaNPV) of Helicoverpa Armigera ...................................... 31
10. Procedure for Mass Production of Cryptolaemus Montrouzieri Mulsant ...................................................................... 35
11. Mass Production of Chilocorus Nigrita Fabricious ...................... 41
12. Mass Production of Scymnus Coccivora Ayyar ........................... 47
13. Mass Production of Anthocorid Bugs ......................................... 53
14. Procedure for Mass Production of Reduviid Predators ............. 57
15. Mass Production of Chrysopid Predators ................................... 65
16. Mass Multiplication Techniques of Queen Apis Mellifera .................................................................................... 75
17. Techniques for Isolation of Fungal Biocontrol Agents Endophytes and Evaluation ........................................................103
18. Methods to Test the Effectiveness of Biocontrol Agents Against Targeted Pathogens .........................................105
19. Isolation of Biofertilizer Microbes from Crops Rhizosphere ....................................................................................107
20. Isolation of Potash, Phosphate and Zn Solubilizing Microbes ....................................................................113
21. Isolation and Diagnosis of Nematodes ......................................117
22. Preparation of Temporary and Permanent Mount for Identification of Nematodes.....................................125
23. Purification of Biological Agents ................................................135
24. Counting of Cell/Spores in Microscope ....................................137
25. Preparation of Liquid Bioformulation of Fungal Biocontrol Agents and Its Self Life Study .................................157
26. Mass Multiplication Techniques Flowchart .............................169
27. References .......................................................................................177
